Within the corridors of the Central Laboratory at the University of Anbar, and as part of the activities of the second day of the practical workshop on Next-Generation Sequencing (NGS), the steps of researchers and interested individuals continued towards new horizons of applied knowledge. This day witnessed a greater depth in dealing with modern technologies, where the practical program focused on the steps of in-silico library preparation and the use of advanced equipment that is considered the beating heart of NGS technology.